Inherited and improved the physical layer and media access control (MAC) layer technology of 802.11, 802.11n wireless protocol improved throughput. However, facing with a complex indoor environment and radio interference around, the wireless throughput will be affected. In this paper, the wireless network throughput of 802.11n with the rate of 300 Mbps and 450 Mbps are measured and compared in an office environment with different bandwidth of 20 MHz and 40 MHz. Whats more, the throughput of the different clients with Android, Windows7 and Windows XP systems are analyzed. The result provides an efficient reference for 802.11n wireless LAN deployment in the building environment.